Coding

Part:BBa_J100131:Design

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2013-09-23)


human IL-6


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 603
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 604
    Illegal PstI site found at 618
    Illegal NotI site found at 7
    Illegal NotI site found at 611
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 604
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 604
    Illegal PstI site found at 618
    Illegal AgeI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23
    Illegal BsaI.rc site found at 597


Design Notes

Internal restriction sites removed for EcoRI, XbaI, SpeI, PstI or BsaI. This insert was synthesized by GeneArt and was cloned into their pMK-T plasmid so we had the BioBrick prefix and suffix included in the basic part sequence. It will be used with Golden Gate Assembly to put downstream of promoter+RBS.


Source

came from GenBank and then was modified.

References